IMPACT OF HYDRO-METHANOLIC SEED EXTRACT OF AZANZA GARCKEANA ON LIPID PROFILES AND OXIDATIVE STRESS IN MALE WISTAR RATS

Abstract

The prevalence of cardiometabolic conditions has risen globally over the years even in the low- and middle income countries. The present study was aimed at investigating the effects of hydromethanolic seed extract of Azanza garckeana on the lipid profile and oxidative stress markers of male wistar rats. A total of 20 wistar rats were separated into four groups of five rats each; including group 1 which served as control. Groups 2, 3, and 4 respectively received a daily dose of 250mg/kg, 500mg/kg, and 1000mg/kg of the extract. The body weight of the animals was taken on day 1 and day 28. The experiment lasted for 28 days, thereafter, the animals were sacrificed and blood samples collected for the determination of lipid profile and oxidative stress markers. The result showed that 1000mg/kg of the extract caused a significant increase in total cholesterol, low-density lipoprotein, and very low-density lipoprotein levels but no significant effect on the triglyceride, and high-density lipoprotein. The percentage of weight gain was significantly reduced compared to control. 250mg/kg and 500mg/kg of the extract did not cause significant changes in the lipid profile parameters. There were also no significant changes in the oxidative stress parameters. This therefore suggests that at higher doses hydro-methanolic seed extract of Azanza garckeana despite causing dyslipidaemia, may have a weight protective effect and would not potentially increase lipid peroxidation in male wistar rats.